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Q: Ion exchange chromatography is the method of choice for the separation of :
  • A. Metals
  • B. Sugars
  • C. Amino acids
  • D. Sterols
Correct Answer: Option C - Ion-exchange chromatography is particularly useful for separating amino acids because they exist as zwitterions (molecules with both positive and negative charges) at different pH levels. This technique exploits the differences in the charge properties of amino acids, allowing them to bind to either cation-exchange or anion-exchange resins depending on their net charge at a specific pH.
C. Ion-exchange chromatography is particularly useful for separating amino acids because they exist as zwitterions (molecules with both positive and negative charges) at different pH levels. This technique exploits the differences in the charge properties of amino acids, allowing them to bind to either cation-exchange or anion-exchange resins depending on their net charge at a specific pH.

Explanations:

Ion-exchange chromatography is particularly useful for separating amino acids because they exist as zwitterions (molecules with both positive and negative charges) at different pH levels. This technique exploits the differences in the charge properties of amino acids, allowing them to bind to either cation-exchange or anion-exchange resins depending on their net charge at a specific pH.