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Q: A substance used for modification of silica gel for reversed phase TLC is :
  • A. Glycerin
  • B. Silicone oil
  • C. Ether
  • D. Benzene
Correct Answer: Option B - Silicone oil is a commonly used substance for modifying silica gel for reversed-phase thin-layer chromatography (TLC). It is applied to the silica gel surface, creating a non-polar layer. This allows for the separation of compounds based on their polarity, with more polar compounds being retained longer on the stationary phase.
B. Silicone oil is a commonly used substance for modifying silica gel for reversed-phase thin-layer chromatography (TLC). It is applied to the silica gel surface, creating a non-polar layer. This allows for the separation of compounds based on their polarity, with more polar compounds being retained longer on the stationary phase.

Explanations:

Silicone oil is a commonly used substance for modifying silica gel for reversed-phase thin-layer chromatography (TLC). It is applied to the silica gel surface, creating a non-polar layer. This allows for the separation of compounds based on their polarity, with more polar compounds being retained longer on the stationary phase.