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Q: The specific test for identifying imidazole rings in amino acids is:
  • A. Pauly's test
  • B. Biuret test
  • C. Millon's test
  • D. Folin's test
Correct Answer: Option A - The specific test for identifying imidazole rings in amino acids is Pauly's test. Pauly's test is a biochemical test for the detection of tyrosine and histidine where the reagent couples with amines, phenols and imidazole groups. The test was discovered by and is named after the German chemist Hermann pauly. Pauly's test is a specific test for proteins containing tyrosine & histidine as the reagent undergoes diazotization with aromatic groups. In this test when formation of red color indicate presence of tyrosine & histidine. The reaction is performed under cold conditions as the diazonium compound can only form at cold temperatures.
A. The specific test for identifying imidazole rings in amino acids is Pauly's test. Pauly's test is a biochemical test for the detection of tyrosine and histidine where the reagent couples with amines, phenols and imidazole groups. The test was discovered by and is named after the German chemist Hermann pauly. Pauly's test is a specific test for proteins containing tyrosine & histidine as the reagent undergoes diazotization with aromatic groups. In this test when formation of red color indicate presence of tyrosine & histidine. The reaction is performed under cold conditions as the diazonium compound can only form at cold temperatures.

Explanations:

The specific test for identifying imidazole rings in amino acids is Pauly's test. Pauly's test is a biochemical test for the detection of tyrosine and histidine where the reagent couples with amines, phenols and imidazole groups. The test was discovered by and is named after the German chemist Hermann pauly. Pauly's test is a specific test for proteins containing tyrosine & histidine as the reagent undergoes diazotization with aromatic groups. In this test when formation of red color indicate presence of tyrosine & histidine. The reaction is performed under cold conditions as the diazonium compound can only form at cold temperatures.