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Q: The mobile phase in Reverse Phase
  • A. Water and petroleum ether
  • B. Water and methanol HPLC comprises ______ and ______.
  • C. Water and carbon tetrachloride
  • D. Water and cyclohexane
Correct Answer: Option B - In reversed-phase chromatography, the mobile phase is very polar water or mixtures of water and methanol. The solvent used to separate components in a liquid sample for HPLC analysis is called the mobile phase.
B. In reversed-phase chromatography, the mobile phase is very polar water or mixtures of water and methanol. The solvent used to separate components in a liquid sample for HPLC analysis is called the mobile phase.

Explanations:

In reversed-phase chromatography, the mobile phase is very polar water or mixtures of water and methanol. The solvent used to separate components in a liquid sample for HPLC analysis is called the mobile phase.